In order to study the dynamic performance of the steel tube flange composite beam bridge, a three-dimensional vehicle-bridge coupled vibration model was established through the finite element software ABAQUS, and implicit dynamics are used to analyze the influence of factors such as road surface roughness, vehicle load, speed, concrete grade and steel content of the upper flange steel tube on the dynamic performance of the tube flange composite beam bridge, as well as the dynamic performance of I-beam and concrete beams equivalent to the vertical stiffness of tube flange composite beams. The result shows that, with the increase of road surface roughness, the dynamic response of tube-flange composite beams increases geometrically, and it is more consistent with the dynamic impact system calculated by the formula in the current the General Code for Design of Highway Bridges and Culverts JTG D60-2015 when the road surface level is B. The vehicle load is linearly related to its deflection. The influence of speed on it is more complicated. When the vehicle speed exceeds 60 km/h, its dynamic response increases nonlinearly with the increase of speed. The concrete grade and steel content in the upper flange affect it the dynamic influence is extremely small. Compared with the equivalent I-beam and equivalent concrete beam, the tube flange composite beam bridge which has a smaller overall elastic modulus is light in weight and has a larger deflection under the same load. The above-mentioned dynamic performance research will provide technical support for vigorously developing this type of bridge in the future.