In order to improve the efficiency of subway travel for wheelchair users, the accessibility of current Beijing subway guidance signage system is used to investigate in this experiment. The experiment selected two factors affecting the accessibility of the guidance, route complexity and the availability of accessible guide signs, as independent variables, and selected interruption, confusion, and time spent in the way-finding as dependent variables. The experimental route were selected by the analytic hierarchy process and quantitative analysis, and participants were recruited to field test the efficiency of using wheelchairs for travel. The results show that complexity has significant effects on interruptions, confusion, and time spent; usability has significant effects on confusion and time spent; and intersections are key locations that constrain wheelchair users" way-finding performance. It is concluded that reducing route complexity and improving the usability of accessible signs at specific intersections can both improve the way-finding efficiency of wheelchair users. According to the experimental results, it is suggested that the complexity and usability remain under the given reference range, in order to maintain a higher efficiency of wheelchair users" travel.