Because natural cracks are not smooth cracks, in the process of identifying cracks by acoustic and electrical imaging, if cracks are regarded as smooth cracks, errors will be generated. Stoneley wave reflection coefficient has high sensitivity to crack width, and stoneley wave is often used to identify cracks. In this paper, physical experiment and numerical simulation are used to study the influence of rough fracture on acoustic response. In the aspect of experiment, the crack roughness experiment of model well is carried out, and the relationship between crack width and attenuation coefficient of rough crack is obtained. Numerical simulation of horizontal crack borehole model is carried out to study the influence of sinusoidal rough crack on stoneley wave. The roughness of crack surface is described by tortuosity. The experimental results show that the attenuation coefficient of stoneley wave will increase with the increase of notch on the crack surface. The simulation results show that the smaller the width of rough crack, the larger the gap between the attenuation coefficient of smooth crack and the same width. The relationship between stoneley wave attenuation coefficient and crack width and tortuosity is established by fitting.