高产IAA绿色木霉工程菌株的构建及其应用
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Q935

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国家重点研发计划(2017YFD0200806);国家自然科学基金(21607169);山东省自然科学基金(ZR202102270296);山东省科学院青年科学(2020QN0019, 2020QN0017);济南市创新团队项目(2019GXRC033)


Construction and Application of Engineering Strains of Trichoderma viride with High IAA Yield
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    摘要:

    农药和化肥的滥用,对人类健康及生存环境构成极大威胁,研制出能够降低化肥用量的的新型肥料十分必要,符合农业可持续发展的新型肥料十分必要。木霉作为新型肥料的常用功能微生物,在农业领域有着广泛的用途。在众多的促生防病机制中,通过产生吲哚乙酸(indole-3-acetic acid,IAA)来促进植株生长是木霉发挥作用的重要方式。为了进一步增强木霉的促生抗病能力,我们在绿色木霉Tv-1511中鉴定并克隆了IAA生物合成途径关键酶色氨酸合成酶(tryptophan synthase,TRPS)的编码基因TvTRPS以及色氨酸脱羧酶(L-Tryptophan decarboxylase,TDC)的编码基因TvTDC,构建了TvTRPS基因和TvTDC基因过表达的绿色木霉工程菌,并研究了其对木霉耐胁迫能力,产IAA能力,植物促生能力等的影响。结果表明,过表达TvTRPS基因和TvTDC基因的木霉工程菌合成IAA的能力显著提高,并且具有更强的耐胁迫能力和促生能力,为绿色木霉在农业生产上的进一步应用奠定了基础。

    Abstract:

    The abuse of pesticides and chemical fertilizers poses a great threat to human health and living environment. It is very necessary to develop new fertilizers that can reduce the amount of chemical fertilizers and meet the sustainable development of agriculture. Trichoderma, as a common functional microorganism of new fertilizer, has a wide range of applications in the field of agriculture. Among many mechanisms of promoting growth and disease prevention, it is an important way for Trichoderma to promote plant growth by producing indole-3-acetic acid (IAA). In order to further enhance the growth promoting effect and disease resistance of Trichoderma viride, we identified and cloned the TvTRPS gene encoding tryptophan synthase (TRPS) and the TvTDC gene encoding L-tryptophan decarboxylase (TDC) in Trichoderma viride Tv-1511, constructed the engineering strain of Trichoderma viride overexpressing TvTRPS and TvTDC, and evaluated their effects on stress tolerance、IAA production and plant growth promotion. The results showed that Trichoderma viride engineering strains overexpressing TvTRPS and TvTDC had the improved ability to synthesize IAA, as well as had stronger stress tolerance and growth promotion ability, which laid a foundation for the further application of Trichoderma viride in agricultural production.

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张豪,李哲,郭凯,等. 高产IAA绿色木霉工程菌株的构建及其应用[J]. 科学技术与工程, 2022, 22(20): 8623-8631.
Zhang Hao, Li Zhe, Guo Kai, et al. Construction and Application of Engineering Strains of Trichoderma viride with High IAA Yield[J]. Science Technology and Engineering,2022,22(20):8623-8631.

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历史
  • 收稿日期:2021-10-14
  • 最后修改日期:2022-04-02
  • 录用日期:2022-03-03
  • 在线发布日期: 2022-08-04
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