Solid natural gas hydrate is a kind of energy storage method with high efficiency and safety. However, it has been limited by harsh generation conditions and slow generation rate. Therefore, hydrate promoters have become the focus of attention in recent years. By comparing the effect of norvaline, alanine and histidine in the normal pressure cyclopentane hydrate system and the high pressure methane hydrate system, the feasibility of a preliminary screening method for evaluating hydrate promoters performance under safer conditions using cyclopentane hydrate has been studied. Compared with alanine and histidine, norvaline in the cyclopentane system had the most efficient promotion effect, which significantly increased the rate of the hydrate formation with the optimal concentration of 0.5wt%. The induction time and the maximum reaction temperature was 9.5 min and 4.5 ℃ respectively. In addition, the emulsifier Span 20 had a good synergistic effect on the norvaline. Similarly, norvaline also had the best promotion performance in the methane hydrate system, effectively enhancing the gas storage capacity of the methane hydrate. It has been found that high initial subcooling led to fast hydrate formation rate and large gas storage capacity. Overall, the cyclopentane hydrate system can be used as a preliminary screening method for high-efficiency amino acid promoters.