GFPT2基因系统发育与功能生物信息学分析
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R363.1

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山西省回国留学项目 (2017-067);国家自然科学基金 (No.: 81700256).


Bioinformatics analysis of GFPT2 gene and its correlation with cardiac hypertrophy
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    摘要:

    GFPT2作为己糖胺代谢重要的转录因子,在动物的多种疾病发生发展中起着重要作用.。利用生物信息学方法对GFPT2蛋白的组分、功能、正选择位点及其分子进化进行了详细的分析。结果表明:13条不同物种氨基酸序列组分分析显示亮氨酸占比最高为9.86%,含量最低的氨基酸为色氨酸 (0.15%)。平均长度为664.8。系统进化树和结构域显示人和猴子的亲缘性最近,其次是大、小鼠,所有氨基酸均包含两个SIS保守结构域。MEME 和REL方法共发现8个正选择位点。GO功能富集分析发现GFPT2主要参与GFPT酶活性、蛋白结合、碳水化合物衍生物结合方面发挥作用等生物学过程。KEGG分析显示差异基因主要参在氨基糖和核苷酸糖代谢,丙氨酸、天冬氨酸和谷氨酸代谢,胰岛素抵抗三条信号通路。并且筛选到与GFPT2相互作用的10个基因:GFPT1、GLUL GNPDA1、GNPNAT1、GPI、HK1、MPI、PPAT、AMDHD2、CAD。本文研究结果可以为GFPT2分子功能的深层次研究提供一定的借鉴作用,对进一步探究由己糖胺代谢导致的代谢异常及心血管疾病治疗具有重要的现实意义。

    Abstract:

    As an important transcription factor of hexosamine metabolism, GFPT2 plays an important role in the occurrence and development of animal diseases. The components, functions, positive selection sites and molecular evolution of GFPT2 protein were analyzed in detail by bioinformatics methods. The results showed that the highest proportion of leucine was 9.86%, and the lowest content was tryptophan (0.15%). The average length was 664.8. Phylogenetic tree and domains showed that human and monkey had the closest genetic relationship, followed by rats and mice. All amino acids contained two SIS conserved domains. Eight positive selection sites were found by meme and rel methods. Go functional enrichment analysis showed that GFPT2 was mainly involved in GFPT enzyme activity, protein binding, carbohydrate derivative binding and other biological processes. KEGG analysis showed that the differentially expressed genes were mainly involved in amino sugar and nucleotide sugar metabolism, alanine, aspartate and glutamic acid metabolism, and insulin resistance. Ten genes interacting with GFPT2 were screened: gfpt1, glul gnpda1, gnpnat1, GPI, HK1, MPI, ppat, amdhd2, CAD. The results of this study can provide a reference for the further study of the molecular function of GFPT2, and have important practical significance for further exploring the metabolism caused by hexosamine metabolism and the treatment of cardiovascular diseases.

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魏思昂,丁志文,冯焱. GFPT2基因系统发育与功能生物信息学分析[J]. 科学技术与工程, 2021, 21(21): 8814-8821.
Wei Siang, Ding Zhiwen, Feng Yan. Bioinformatics analysis of GFPT2 gene and its correlation with cardiac hypertrophy[J]. Science Technology and Engineering,2021,21(21):8814-8821.

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  • 收稿日期:2020-12-30
  • 最后修改日期:2021-05-21
  • 录用日期:2021-03-16
  • 在线发布日期: 2021-08-11
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