Abstract:As an important transcription factor of hexosamine metabolism, GFPT2 plays an important role in the occurrence and development of animal diseases. The components, functions, positive selection sites and molecular evolution of GFPT2 protein were analyzed in detail by bioinformatics methods. The results showed that the highest proportion of leucine was 9.86%, and the lowest content was tryptophan (0.15%). The average length was 664.8. Phylogenetic tree and domains showed that human and monkey had the closest genetic relationship, followed by rats and mice. All amino acids contained two SIS conserved domains. Eight positive selection sites were found by meme and rel methods. Go functional enrichment analysis showed that GFPT2 was mainly involved in GFPT enzyme activity, protein binding, carbohydrate derivative binding and other biological processes. KEGG analysis showed that the differentially expressed genes were mainly involved in amino sugar and nucleotide sugar metabolism, alanine, aspartate and glutamic acid metabolism, and insulin resistance. Ten genes interacting with GFPT2 were screened: gfpt1, glul gnpda1, gnpnat1, GPI, HK1, MPI, ppat, amdhd2, CAD. The results of this study can provide a reference for the further study of the molecular function of GFPT2, and have important practical significance for further exploring the metabolism caused by hexosamine metabolism and the treatment of cardiovascular diseases.