Use of a noncytotoxic Clostridium perfringens as an oral HIV vaccine vector to express high levels of HIV p24 protein,the entire HIV p24 ORF was amplified by PCR, then digested and ligated with plasmid pJRC200 ,which contained a Clostridium perfringens DNA and had been removed most of the CPE ORF. The recombinant cp24 was transformed into a noncytotoxic Clostridium perfringens strain ATCC3624 (cpe-negative, type A) to be highly expressed during sporulation. It is developed a recombinant plasmid pJRC200, which contained HIV p24 ORF. HIV p24 ORF was expressed successfully. A highly expressed HIV p24 strain ATCC3624 was abstained. This work will be a base for the study of the use of Clostridium perfringens as an oral HIV vaccine.