Objective: As a common N-glycosylation, core fucosylation is an important post-translational modification mechanism, which can quickly change the biological properties of proteins and the activity of enzyme. Core fucosylation plays an important role in mammalian cell recognize, proliferate and metabolism. In human beings, α1,6-Fucosyltransferase (FUT8) is the only protein which possesses core fucosyltransferase activity. The present study aims to analyze the structure and expression of fut8 gene coding FUT8 protein. In addition, amino acid sequence, three-dimensional structure information and the protein interaction networks of FUT8 protein were analyzed by using bioinformatics to explore its functions and its role in related disease. Methods: The structure, location, and expression regulation characteristics of fut8 gene were analyzed by using GenBank database of NCBI website. The physical and chemical properties, transmembrane domain, three-dimensional structure, protein interaction networks and pathways of FUT8 protein were analyzed by GenPept and PDB databases, and ProtParam, PPI and KEGG tools etc. Results: The human fut8 gene is located at 14q23.3 while the longest transcription product is 3895 bp, encoding a protein with 575 amino acids. The product protein, FUT8, is weakly alkaline and hydrophilic, with a transmembrane segment from the inside to the outside. The main element of FUT8 secondary structure is alpha helix, which forms 4 domains. FUT8 interacts with a variety of proteins and receptors, catalyzes the formation of core fucosylation of the protein. Sequence alignment shows that FUT8 has multiple conserved domains and is highly conserved in species evolution. Conclusion: The physicochemical properties, theoretical transmembrane domain information, three-dimensional structure, protein pathways and interaction networks of human FUT8 protein have been successfully obtained by multiple bioinformatics databases and tools, which provide theoretical basis for further study of FUT8 in the fields of obesity and related diseases, tumor metastasis, inflammation, dysimmunity and hematopoiesis abnormal.