Objective: Study on adsorption characteristics and optimum extraction technology of total flavonoids from Astragalus membranaceus by LSA-10 Resin. Methods: Through the static adsorption and desorption experiments of 7 kinds of resins, the selection of macroporous adsorption resins was determined, the adsorption kinetics and adsorption isotherms were investigated. The process of flavonoid separation by this resin was optimized. Results: Kinetic studies show that the pseudo-second-order model of the adsorption process can better fit the adsorption process of the LSA-10 resin than the pseudo-first-order model. The adsorption isotherm studies show that the adsorption of the total flavones of astragalus by the LSA-10 resin is most consistent with the Langmuir isotherm adsorption equation. Optimal conditions for the separation of total flavonoids in Astragalus membranaceus by LSA-10 macroporous adsorption resin is: loading concentration of astragalus extract 0.5 mg/mL, loading volume 4 BV(bed volume), loading flow rate 3 mL/min, eluent dosage 8 BV, 60 % ethanol was eluted at a rate of 2 BV/h. Conclusion: The adsorption capacity of LSA-10 resin for the total flavonoids of Astragalus Membranaceus was significantly greater than that of other components.