Combined with survey data and GIS technology, Johnes classic export coefficient models were used to estimate the agricultural non-point source pollution loads of TN and TP in the upstream of Qingshui river in 2000, 2005, 2010 and 2015, and its temporal and spatial variation characteristics and pollution sources were analyzed.The results showed that the export loads of TN and TP in the four periods decreased significantly, and the former was 9.2~9.8 times higher than the latter.The influence of different pollution source types on the contribution rates of TN and TP loads was similar, and land use was the most critical factor, which was shown as cultivated land > grass land > forest land.From the spatial change trend, the distribution characteristics of TN and TP pollution loads intensity were similar, and the overall distribution was uneven. The high loads areas were concentrated in the plain and hilly areas with small slope on both sides of the Qingshui River tributary system, while the low loads areas were mainly distributed in the water conservation areas with less cultivated land, and the TN loads intensity is about 10 times of TP.Analyzed the pollution sources from the perspectives of land use,livestock breeding and rural life,the results showed that cultivated land, especially economic crops such as vegetables, was the main source of agricultural non-point source pollution, and livestock breeding and residents' life were the important components of non-point source pollution.