The effect of temperature on the property of limestone is involved in the field of LNG storage, nuclear engineering construction, underground gas and geothermal resource development. Thus, thermal and mechanical properties of limestone affected by temperature were needed to be studied. By using the self-developed force-temperature synchronous loading test chambers, the experiments were carried on regarding limestone subjected to coupling effect of temperature changing and loading from room temperature to high temperature of 300℃ and to low temperature of -165℃. The temperature and force-induced strain output were separated by the in-situ compensation method. Therefore, the coefficient of linear thermal expansion (CLTE) of limestone and its variation trend with temperature were obtained. The stress-strain relationship curves at different temperatures were established through the loading test, from which the variation of the elastic modulus of limestone with temperature was obtained. The results show that: When the temperature cooling from 0℃ to -165℃, the linear expansion coefficient of limestone gradually decreases by an average of 86%, and its elastic modulus decreases by 10% compared with 0℃. When the temperature is elevating from 30 ℃ to 300 ℃, the linear expansion coefficient of limestone increases by an average of 58%. And the elastic modulus decreases significantly with dropping by 60 % .