黄芪甲苷下调托样受体4抑制心肌梗死心肌组织损伤的作用
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R285. 5

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国家自然科学基金


Inhibition Effects of Astragaloside IV on Myocardial Injury in Rats with Myocardial Infarction by Regulating Toll-like Receptor 4
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The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)

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    摘要:

    分析黄芪甲苷调控托样受体4(Toll-like receptor 4 (TLR4), )抑制心肌梗死心肌组织损伤的作用并探讨其作用机制。将Wistar大鼠按随机数字表法分为假手术(Sham)组、二甲基亚砜(DMSO)组、模型(Model)组和黄芪甲苷治疗(AS-IV)组,每组均为10只。Model组采用经典的左冠状动脉结扎术复制心肌梗死模型,Sham组手术处理但没有进行结扎处理。AS-IV组将AS-IV溶于1%的DMSO中,20 mg·(kg·d)-1腹腔注射。Sham组和Model组给予等量的生理盐水,DMSO组给予等量含1%DMSO的生理盐水。给药方式均为腹腔注射,每日1次,连续14 d。采用HE染色法分析心肌组织病理变化,TUNEL法检测心肌细胞的凋亡程度。逆转录PCR(RT-PCR)检测心肌细胞TLR4 mRNA的表达,免疫印迹法和免疫组化法检测心肌心肌细胞TLR4蛋白的表达。结果表明:AS-IV治疗可显著减轻心梗大鼠心肌组织的坏死程度,降低炎症细胞的数量,减少疤痕组织的增生,显著减轻心肌细胞的凋亡,下调心肌组织中TLR4 mRNA和蛋白的表达。可见AS-IV通过下调心梗大鼠心肌组织中TLR4样受体的表达而对抗心梗后心肌组织的损伤。

    Abstract:

    In order to investigate the inhibitory effect of Astragaloside IV(AS-IV) on myocardial injury in rats with myocardial infarction by regulating toll-like receptor 4 (TLR4). Wistar rats were randomly divided into sham operation (Sham), dimethyl sulfoxide (DMSO), model and AS-IV groups with 10 rats in each group. The left coronary artery ligation was used to replicate the myocardial infarction model in the model group, but no ligation was performed in the sham and DMSO groups. In AS-IV group, AS-IV was dissolved in 1% of DMSO and 20 mg·(kg·d)-1 intraperitoneally. The sham group and model group were given the same amount of normal saline, and the DMSO group was given the same amount of saline containing 1%DMSO. All the rats were administered intraperitoneally 1 times a day for 14 consecutive days. The pathological changes of myocardial tissue were analyzed by HE staining, and the apoptosis of cardiomyocytes was detected by the TUNEL method. Reverse transcription quantitative PCR (RT-qPCR) was used to detect the expression of TLR4 mRNA in cardiomyocytes, western blotting and immunohistochemistry were used to detect the expression of TLR4 protein in cardiomyocytes. The results show that AS-IV treatment can significantly reduce the degree of myocardial necrosis, reduce the number of inflammatory cells, reduce the proliferation of scar tissue, significantly reduce the apoptosis of myocardial cells, down-regulate the expression of TLR4 mRNA and protein in myocardial tissue. It is concluded that AS-IV can inhibit myocardial injury by down-regulating the expression of TLR4-like receptor in myocardial tissue of rats with myocardial infarction.

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. 黄芪甲苷下调托样受体4抑制心肌梗死心肌组织损伤的作用[J]. 科学技术与工程, 2019, 19(11): .
. Inhibition Effects of Astragaloside IV on Myocardial Injury in Rats with Myocardial Infarction by Regulating Toll-like Receptor 4[J]. Science Technology and Engineering,2019,19(11).

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  • 收稿日期:2018-12-05
  • 最后修改日期:2019-01-31
  • 录用日期:2019-02-13
  • 在线发布日期: 2019-04-25
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