Based on the more and more serious influence of low frequency noise of urban substation, this paper introduces the concept and research method of sound quality evaluation to evaluation of urban substation noise. Collecting the whole-indoor urban substation noise for 110kV, 220kV and 500kV, and the grading method is used to carried out the subjective test of annoyance quality of the substation noise, and several psychoacoustical parameters are calculated for the tested acoustic samples. the prediction model of annoyance quality of urban substation noise is established by the correlation analysis and regression analysis. The results show that the annoyance of urban substation noise has a relatively high correlation with A sound level, loudness, roughness and fluctuation, but has a low correlation with sharpness. The subjective prediction model of annoyance mainly depends on A sound level and roughness, and the weight of the A sound level is significantly higher than the roughness. By evaluating the annoyance quality of urban substation noise, it can provide a concrete and new idea and way for the substation noise control.