11批次白芷药材的HPLC和GC指纹图谱研究
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南阳理工学院

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R917

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白芷药材的质量控制研究(河南省张仲景方药与免疫调节重点实验室2017年度开放资助项目)


Study on HPLC and GC Fingerprint of the 11 batch of Angelica dahurica
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NanYang Institute of Technology

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    摘要:

    摘要 目的:建立白芷GC及HPLC指纹图谱,为白芷药材的质量评价提供参考。方法:运用HPLC及GC法对白芷药材水提液及挥发油进行指纹图谱研究,HPLC采用Diamonsil C18 (250mm×4.6mm, 5μm) 色谱柱,A相:乙腈,B相:0.2%冰醋酸溶液(三乙胺调pH5.0);柱温30℃,进样量10μL,波长245nm;流速:1mL/min。乙腈比例为0-35min:10-25,35-55min:25-58,55-70min:58-80,70-75min:80-100。GC采用HP-5毛细管气相色谱柱(30 m x 0. 25 mm x 0. 25um),采用程序升温模式,升温程序为55℃(10min)以4℃/min的速度线性升温至250℃(8min)。结果:通过建立11批白芷药材HPLC及GC指纹图谱,对白芷药材的质量进行控制,HPLC实验各批次之间相似度0.9以上,GC实验中各批次之间相似度高于0.8,各批次相似度较佳。结论:首次建立了11批白芷药材HPLC及GC指纹图谱分析方法,该法稳定可靠、重复性好,对白芷药材的质量控制提供了全面可靠的科学依据。

    Abstract:

    [Abstract] Objective: To establish GC and HPLC fingerprint of Angelica dahurica and provide reference for quality evaluation of Angelica dahurica. Methods:Using HPLC and GC method of water extract of Radix Angelicae dahuricae and volatile oil were studied using Diamonsil C18 fingerprint, HPLC (250mm * 4.6mm, 5 m) column, acetonitrile, A: B: 0.2% acetic acid solution (three pH5.0 triethylamine); column temperature of 30 DEG C, sample volume 10 L wave length 245nm; flow rate: 1mL/min. The ratio of acetonitrile to 0-35min:10-25, 35-55min:25-58, 55-70min:58-80, 70-75min:80-100. GC using HP-5 capillary gas chromatography column (30 m x mm x 0 25um), using the temperature programmed mode, heating program for the temperature of 55 DEG C (10min) at a temperature of 4 /min linear heating up to 250 DEG C (8min). Results: Through the establishment of the 11 batch of Baizhi HPLC and GC fingerprint, quality control of Radix Angelicae dahuricae, similarity between HPLC experimental batches of more than 0.9, the similarity between each batch experiment in GC is higher than 0.8, each batch of similarity is better. Conclusion: The method of HPLC and GC fingerprint analysis of 11 batches of Radix Angelicae dahurica was established for the first time. This method is stable, reliable and reproducible. It provides a scientific basis for the quality control of Radix Angelicae dahurica.

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张超云. 11批次白芷药材的HPLC和GC指纹图谱研究[J]. 科学技术与工程, 2018, 18(3): .
张超云. Study on HPLC and GC Fingerprint of the 11 batch of Angelica dahurica[J]. Science Technology and Engineering,2018,18(3).

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  • 收稿日期:2017-06-29
  • 最后修改日期:2017-08-31
  • 录用日期:2017-09-20
  • 在线发布日期: 2018-02-27
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