The fresh natural rubber latex was coagulated by lysozyme and alkaline protease, and the vulcanization characterizations and mechanical properties of natural rubber were investigated respectively. The natural rubber obtained by coagulating latex with lysozyme bears high protein content and low vulcanization rate and the vulcanizate exhibits high crosslinking density, mechanical properties, Tg value and compression heat build-up. While the latex was coagulated by alkaline protease, the hydrolysis of proteins might reduce the protein content. The vulcanization process of natural rubber can be accelerated hereby but the crosslinking density and mechanical properties of natural rubber vulcanizate were decreased.