In order to investigate the fire evacuation performance of subway train, the paper firstly analyzes the poisonous gas and visibility in subway train in case of fire and confirms the minimum transfer time that a single carriage fully loaded with passengers shall satisfy by means of Pyrosim fire numerical simulation software and in combination with the physical and chemical parameters of real materials. Subsequently, the real evacuation scene in subway in case of fire is simulated and the large-scale evaluation experiments are carried out with a view to explore the actual transfer time for crowd and the relevant points of attention. Finally, a comparative analysis between platform evacuation and tunnel evacuation is carried out through Pyrosim evacuation simulation software.