利用miR-17的腺病毒载体抑制肺癌细胞系增殖
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沈阳军区总医院,呼吸与重症医学科

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R282. 71;R285.5

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Expression of miR-17 via adenoviral vector suppress the proliferation of A549 cells
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    摘要:

    [摘要] 背景:miR-17是肺癌的新型调节因子,有可能在肺癌发生和进展中发挥作用,但其功能尚不完全清楚。目的:构建miR-17的腺病毒表达载体,检测miR-17对A549细胞体外增殖、锚定非依赖性生长、侵袭和转移的调控作用。方法:利用miR-17的腺病毒载体(Ad-miR-17)在A549细胞中过表达miR-17后,使用MTT实验检测miR-17对NSCLC细胞系A549增殖作用的影响;软琼脂成集落实验检测miR-17对A549细胞锚定非依赖性生长的作用,使用Trans-well实验检测A549细胞的体外侵袭/转移作用(in vitro invasion/in vitro migration)。在此基础上共转染miR-17的inhibitor确定miR-17作用的特异性。结果:miR-17能够显著下调A549细胞的增殖、锚定非依赖性生长、体外侵袭/转移作用。共转染miR-17的inhibitor能够阻断miR-17的作用。结论:miR-17能够抑制A549细胞增殖。

    Abstract:

    [ABSTRACT] Background: MicroRNA-17 (miR-17) may participate in the regulation of human lung cancer regulation or progress. However, the detailed function and mechanisms of miR-17 are largely unknown. Objective: To construct the adenoviral vector of miR-17 and examine its roles in lung cancer cells’ proliferation, anchorage independent growth, in vitro invasion and migration. Methods: Full length sequence of miR-17 was cloned into recombinant adenovirus vectors. A549 cells were infected with adenovirus vectors (Ad-miR-17 vectors). The proliferation, anchorage-independent growth in vitro invasion or migration of A549 cells were determined by MTT-assays, soft agar-assays or trans-well assays. The specificity of miR-17 function was confirmed by transfection of miR-17’s inhibitor. Results: Infection of Ad-miR-17 significantly suppressed the proliferation, anchorage-independent growth, in vitro invasion or migration of A549 cells. Co-transfection with miR-17 inhibitor disrupted the effect of miR-17 on A549 cells. Conclusion Expression of miR-17 via identified adenoviral vector suppresses lung cancer cells proliferation.

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刘佳. 利用miR-17的腺病毒载体抑制肺癌细胞系增殖[J]. 科学技术与工程, 2016, 16(31): .
刘佳. Expression of miR-17 via adenoviral vector suppress the proliferation of A549 cells[J]. Science Technology and Engineering,2016,16(31).

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  • 收稿日期:2016-06-21
  • 最后修改日期:2016-10-30
  • 录用日期:2016-08-11
  • 在线发布日期: 2016-11-09
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