Background: Meis 1（Myeloid ecotropic viral integration site 1）would be a potential tumor suppressor in some kinds of human cancer. There is not a universal model of MEIS1. Objective: To identify the potential application of Meis 1 on NSCLC cells’ sensitivity to anti-tumor agents. Methods: The MEIS 1 protein was overexpressed via recombinant adenovirus vectors. The effect of Meis 1 on proliferation of anchorage-independent growth of A549 was determined by MTT or soft agar assays. Next, the MTT-assays were performed in A549, Calu3, H460, H358 or A549/ADR cells to declare the effect of Meis 1 on sensitivity of cells to anti-tumor agents. The inhibition rates or IC50 values were calculated. Results: Overexpression of MEIS 1 attenuated the proliferation of A549 cells, and up regulated the effect of anti-tumor agents: Gefitinib, Sunitinib, Gemcitabine and Paclitaxel. Meis 1 also overcame the MDR process of NSCLC cell. Conclusion By identified the roles of Meis 1 in NSCLC cell lines; we demonstrated that expression of Meis 1 would be a potential application in the reversion of multi-drug resistance process of NSCLC.