Objective: To observe the influence of the method of nourishing the liver and regulating blood and the formula on express of 5-HT、NF-κB and C-fos in PAG of cerebral aqueduct of the rat model of nitroglycerin type migraine.Method: 60 SD rats were divided into 6 group:blank group、model group、controlled group、high dose of decoction group、mid-dose of decoction group and low-dose of decoction group. The decoction and normal saline was given to relevant group by gavage for 14 days. After the last gavage,the migraine rat model was copied by subcutaneous injection of nitroglycerin. To observe the express of NF-κB of PAG of migraine rat by the method of Western bolt,and to observe the express of 5-HT、C-fos by the method of immunohistochemistry.Result:It decreases the express of NF-κB、C-fos in PAG of the nitroglycerin migraine rat model and increases the express of 5-HT in PAG of the rat model by using the decoction of RouGanYuPian preventively.It has the same effect by taking the same dose of this decoction and flunarizine.(P＞0.05)It is better than flunarizine group by taking high-dose.Conclusion: (1)The express of NF-κB in PAG of the nitroglycerin migraine rat model is increased. The decoction of RouGanYuPian can inhibit theNF-κB code pathway of the nitroglycerin migraine rat model , decrease the express of C-fos which is downstream target gene and relieve the neurogenic inflammation.The effect has some dose-effect relationship in some dose range.(2)The express of 5-HT in PAG of the nitroglycerin migraine rat model is decreased. The decoction of RouGanYuPian can improve the express of 5-HT in PAG of the nitroglycerin migraine rat model and decrease the hurt of pain. The effect has some dose-effect relationship in some dose range.