To identify the endophytic fungus DL06 strain from DecaisneaSfargesii and study its secondary metabolites. The strain DL06 was identified as Penicillium chrysogenum by morphological observation and ITS sequence analysis . The fermentation products were isolated and purified by manifold chromatography methods, and their structures were elucidated by physicochemical properties and spectral analysis . Eight compounds were obtained and determined to β-sitosterol(1), 6,8-dihydroxy-3-methylisocoumarin(2), monomethylsulochrin(3), Questin(4), 2-furoic acid(5), 5-hyroxymethyl furfuran-3-carboxylic acid(6), 3-hydroxy-3-(methoxycarb onyl) pentanedi-oic acid(7) and succinic acid(8), Compound(2), (3), (6), and (7) were isolated from the fermentation products of Penicillium chrysogenum for the first time. An MIC method was used in antibacterial activity test, the result show compound (3) has a strong antibacterial activity .