For the purpose of deeply learning about the mechanism of microorganism enhanced oil recovery and building up the mathematical models of microorganism reaction kinetics, exactly based on the reservoir experiment data, the microorganism reaction kinetics research carried out in special reservoir condition, taking a strain of hydrocarbon degrading bacteria as research object. The bacteria named wj-1, identified as pseudomonas aeruginosa and filtrating from Mongolia Lin oilfield, can produce high concentration of biosurfactant rhamnolipid through feeding on oil as carbon source. Based on equation logistic and equation luedeking-piret, kinetics models of bacteria growth, metabolite synthesis and substrate consumption were built up. Model parameters gained through nonlinear fitting in built up models by software origin 8.0. And experiment values and predicted values from models could well matched, indicating that the models could well predict microbic reaction process in the reservoir condition.