Sp1调控FoxM1在卵巢癌中的表达
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第四军医大学西京医院妇产科,第四军医大学西京医院妇产科,第四军医大学西京医院妇产科,第四军医大学基础部细胞生物学教研室,第四军医大学基础部微生物学教研室,第四军医大学西京医院妇产科

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R737.31

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国家自然科学基金项目(面上项目,重点项目,重大项目)


Sp1 regulates the expression of FoxM1 in ovarian cancer ZHAO Jing1, WU Juan2 , YANG Li-na2,LI Yu3*,ZHANG Fang-lin2*,YANG Hong1*
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The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)

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    摘要:

    目的 探讨Sp1调控转录因子FoxM1在卵巢癌中的表达。方法 免疫组织化学染色验证FoxM1在卵巢癌组织中高表达,Spearman,rho秩相关分析Sp1与FoxM1在卵巢癌组织中的表达相关性;双荧光素酶报告系统检测Sp1对FoxM1的调控作用;Real-time quantitative RT- PCR和蛋白免疫印迹技术验证Sp1对FoxM1的调控作用。结果FoxM1在大于60%的卵巢癌组织中呈阳性表达(阳性率33/42≈79%),并且与Sp1的表达显著相关(r=0.809,P<0.01);Sp1可以结合并激活Foxm1启动子(P<0.05);上调Sp1表达可以激活FoxM1在卵巢癌中的表达(P<0.05),而干扰Sp1后可以下调FoxM1的表达(P<0.05)。结论 Sp1参与调控增殖相关转录因子FoxM1在卵巢癌中的表达。

    Abstract:

    Objective This study aims to explore Sp1 regulate the expression of transcription factor FoxM1 in human ovarian cancer.Methods Immunohistochemical staining was used to affirm over-expression of FoxM1 in human ovarian cancer and Spearman’s rho was calculated to analyze the correlation between FoxM1 expression and Sp1 expression.Dual-luciferase reporter assay system was utilized to test Sp1 regulate expression of FoxM1. Real-time quantitative RT-PCR and Western-blot were exerted to validate regulatory effect of Sp1 on FoxM1.Results Over 60% of the ovarian cancer tissues expressed high levels of FoxM1(Positive rate was 33/42≈79%),which had a significant positive correlation with the expression of Sp1 in these ovarian cancer samples(r=0.809,P<0.01).Sp1 was involved in activation of the FoxM1 promoter(P<0.05). Overexpression of Sp1 can activate expression of FoxM1 in ovarian cancer(P<0.05), and interferenced expression of Sp1 downregulated the expression of FoxM1(P<0.05).Conclusion Sp1 may be involved in regulation of expression of proliferation-associated transcription factor Foxm1 in human ovarian carcinoma.

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赵静,吴娟,杨丽娜,等. Sp1调控FoxM1在卵巢癌中的表达[J]. 科学技术与工程, 2014, 14(28): .
zhao jing,,,et al. Sp1 regulates the expression of FoxM1 in ovarian cancer ZHAO Jing1, WU Juan2 , YANG Li-na2,LI Yu3*,ZHANG Fang-lin2*,YANG Hong1*[J]. Science Technology and Engineering,2014,14(28).

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  • 收稿日期:2014-05-14
  • 最后修改日期:2014-06-04
  • 录用日期:2014-06-19
  • 在线发布日期: 2014-10-08
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