Abstract:Phosphodiesterases (Pde) as an enzyme plays important role in G protein signaling. Pde has been reported in Schizosaccharomyces pombe and Magnaporthe oryzae, however, it has not been reported in Colletotrichum higginsianum, which can infect many cruciferous plants. Based on the typical Pde sequence have been reported in Saccharomyces cerevisiae, to search Pde protein sequence from the protein databases of Colletotrichum spp. with the Blastp as well as the use of words, and to search the conserved domain in the SMART online. Meanwhile, bioinformatics analysis have made including the physicochemical properties, hydrophobic, signal peptide, trans-membrane domain structure, hydrophobic location and the secondary structure. In addition, analysis of genetic relationships through comparative Pde in C. higginsanum with homologous sequences of other species, in order to carry out the research including location and function of PDE, and provide strong theoretical foundation to further control pathogens for developing new drug targets with inhibiting G protein signaling pathways.