By connecting the T vector and the egfp gene from plasmid pSEBG to construct the plasmid pZTE, then the egfp gene of the plasmid pZTE was inserted into multiple cloning sites of Kpn I and Sal I in the suicide vector pTnMod-OGm to construct the plasmid pTE-OGm. Last, the transposon elements of egfp gene in pTE-OGm translocated into the chromosome of the strain Xanthomonas Campestris L1, so that the strain Xanthomonas Campestris L1 obtain stable green fluorescent marker, for better to trace the migration and foundation of Xanthomonas Campestris L1 in MBR reactor for further.