Objective: The purpose of the study is to prove the effectiveness of ultrasonography in deep inferior epigastric perforator (DIEP) investigation, and to look for a criteria for choosing the preferable perforator. Methods: 52 cases of breast reconstruction using DIEP underwent preoperative ultrasound inspection. The location where the perforators penetrate the anterior rectus sheath, and the caliber as well as the blood flow information were recorded and then analyzed, to determine the preferable perforator for surgery. Results: The perforator display rate was 100% in all 52 cases, with 3 vessels on each side of the lower abdomen in average. These perforators emerged at the range (5.7±2.09)cm radius below navel. The calibers of perforators suitable for microsurgery were （0.95±0.32）cm on average, with a pulse stream velocity(PSV) of 16.4cm/s and resistant index(RI) of 0.7. The rate which the ultrasonic preferable perforator adopted by the surgeon was 92.31%. Conclusion： Ultrasonography is applicable in perforator preop-investigation, and will play an important role in preferable perforator selection.