Objective To evaluate the protection performances of a new oxygen supply scheme for two pilots against hypoxia at high altitude. Methods Totally 4 rounds of test were conducted in hypobaric chamber. Two dummies were applied in the tests of：①generating performance and supply performance of Molecular Sieve Oxygen Generating System; ②supply performance of gas mixture; ③supply performance of pure oxygen; ④supply performance of pressurized oxygen. Results The oxygen partial pressure provided by the new oxygen supply scheme was over 20.0 kPa (under 12.0 km) and over 16.0 kPa (above 12.0 km)．The inspiration resistance was respectively less than 490 Pa and 578 Pa (above 1.8 km), corresponding to the dummies` respiration ventilation volume of 20 L/min and 30 L/min. Conclusion The new oxygen supply scheme can provide protection against hypoxia for two pilots from grourid to 18.0km.