For those aerodrome areas where collision between aircraft may happen, based on the Airport Surveillance Radar (ASR) data, the taxi tracks of aircraft in the airport maneuver area were extracted. The method of judging whether the taxi route and taxi time of any two aircrafts which go through the same taxiway is overlapped, whether the absolute value of the difference of headings and the separation satisfy the constraint condition of the hot-spot identification in airport was proposed. Moreover, the hot-spot level standard according to the ratio of the average number of collision flights was proposed. Furthermore, the airport conflicts hot-spot identification and level ranking system was developed, with which the hot-spots of an airport Southeast China were identified and ranked. And the results are consistent with the experts’ feedback opinions and actual operation situation, which verified the rationality of conflict hotspots identification approaches and level ranking criteria. This work can remind the pilots pay special attention to the dangerous area, reduce or completely eradicate the ratio of runway incursion and ground collisions, and enhance the safety operational level of aerodrome effectively.