Denitrifying phosphorus removing bacteria was enriched and cultured using the A / O process activated sludge from a wastewater treatment plant in a set of sequencing batch reactor (SBR)for wastewater of low carbon. The system was operated under the condition of anaerobic-aerobic-precipitation-discharge for 10 cycles in the first stage, and the condition of anaerobic-aerobic-anoxic- aerobic -precipitation-discharge were used for 40 cycles in the second stage. Results showed removal of PO_43－-P , NH₄₊-N and COD was 94% ,78% and 86%respectively when the effluent concentration was below0.4mg/L,3mg/L and 20mg/L respectively, which indicated that enrichment and cultivation of denitrifying phosphorus removing bacteria in a set of SBR was feasible.