The human gut microbiota has great importance to human health. We used eLSA algorithm to analyze human gut microbiota time series under antibiotic perturbation．The results showed that the human gut microbiota responded to antibiotic perturbation with both changes in global associations and in local and/or time-delayed associations; Human gut microbiota under repeated antibiotic perturbation, perturbation associations part disappear or emergence some new associations, means antibiotic perturbation can be change the original status of gut microbiota; Individual gut microbiota are different in their response to antibiotics, which may signify the possibility of different patient response to the same antibiotic treatment and imply personalized antibiotic treatment is a direction of further research.