Based on the AEO takeoff ground acceleration segment calculation method of civil transport airplane, presents the unstick point estimation means,foundation the AEO takeoff ground acceleration segment computation module.Take some airplane for example, analysis and calculates the AEO takeoff acceleration segment data ,and compares the results to the results measured during flight test .It provens that ,the calculation method is right and reliable ,also more accuaracy than academic prediction results,this method can be used for amending the disigner’s conception datas,and for another airplanes’ calculation.