Because of the uncertainty of the position and pronunciation angle, auscultation sound of TCM collection methods can not get accurate sound amplitude, so this paper presents an auscultation sound acquisition and improvement method, by adding a specific frequency with fixed sound intensity reference sound as the normalized standard. This method uses the principle of calibration of sound intensity in patients with voice owned by one oriented to the same location of the sound signal amplitude value, thereby eliminating the uncertainty brought from the sound acquisition and pronunciation angle. The device is simple to achieve and the operation is also simple, error is less than 6% after normalizing and the method has been used in research work.