Objective：To generate a serious of mutated CUEDC2 expression clones, and to testify the expression of them in BL21 (DE3) and Sf9 cells. Methods：The primers were designed according to the CDS of cuedc2. A serious of cuedc2 mutants were cloned to expression vectors such as pFastBac1-Flag-N, pFastBac-HTA and pET28a and transformed into the host cells E.coli BL21(DE3) and DH10Bac respectively. The expressed CUEDC2 mutant proteins in BL21(DE3) were purified by the His-tag affinity chromatography and tested by SDS-PAGE. After isolation of recombinant bacmid DNA and transfection of Sf9 cells with them, the recombinant proteins were testified by Western-Blot. Result: The CUEDC2 mutants were successfully cloned to three expression vectors and expressed in E.coli BL21 (DE3) and Sf9 cells. Conclusion: The recombinant proteins were expressed in E.coli and Sf9 cells, which would be useful for the functional and structural research of CUEDC2.