The paper designs the based-on LabVIEW vibrating signal data acquisition system of oilfield water flooding pump, which is used to be more intuitive and convenient to monitor the operation status. This paper describes the overall design and hardware systems, software design ideas, a brief introduction of several major modules of the system design and composition. Base on the collection of the signal of water flooding pump, The system can Real-time analyse the time-domain, frequency domain information, and display diagnostic results through the fault diagnosis system. The testing and feedback of water flooding pump servicing proof that the system we present is reliable for data measuring and accurate for fault diagnose , and convenience for utilization.