[Abstract] To investigate the relationship between levels of fecal calprotection in the inflammatory bowl disease(IBD) of activated and inactivated by ELISA quantitative analysis． A total of 52 fresh fecal samples were collected from the patients with IBD and the healthly volunteers，including 24 cases of UC， 8 cases of CD， and 20 healthy volunteers．EK-CAL was used to quantitatively determine the level of feca1 calprotection．Mayo active index adoptied to evaluate the activity of IBD．the score equal or higher than 3 considered to be active IBD. The level of fecal calprotection was 77.15±160.9 g/g in healthy volunteers, 956.13±488.36g/g in active IBD，337.06±390.52 g/g in inactive IBD， respectively． There was significanted difference between the IBD compared with the healthy volunteers(P< 0.001)． When IBD became active, the level of fecal calprotection increased significantly compared with the inactive IBD and healthy volunteers, respectively (P < 0.05)．When the upper limit was 450.3ug/g,,the sensitivity and specificity of fecal calprotection in differentiating the active IBD was 75% and 90% ,respectively．As a non-invasive method, fecal calprotection is the marker for evaluate IBD activity ,it was have a clinical significance.