For understanding characteristics of ore-forming fluid and source of mineral-forming material of Shaling gold deposit, isotopic properties of hydrogen, oxygen and sulfur and the homogenization temperature of fluid inclusions sampled from drilling of the deposit were investigated by laser Raman analysis and isotopic measurement. The homogenization temperature of fluid inclusions are range in 141～400℃ with the average value of 261℃; the salinity is 1.02%～12.29% NaCleq with the average value of 5.75%NaCleq, and the density is 0.62～1.05 g/cm3 with the average value of 0.80g/cm3. The main components of the fluid inclusions are CO2, H2O, CH4.The temperature, salinity and gas-liquid ratio of the fluid inclusions in different ores regularly vary with its occurred location, the homogenization temperature decreases and the salinity increases from beresitization cataclasite occurred at the center of the frature, to beresitization granite occurred at the outermost of the fracture. The δ34S of Shaling gold deposit is 8.2‰～10.60‰ and its average value is 9.94 ‰; the δ18O of quartz is 10‰～13.8‰, the δ18OH2O is 0.46‰～6.46‰, and the δD is -70.6‰～-89.7‰. The ore-forming fluid of this deposit are the H2O-CO2-NaCl±CH4 with medium-low temperature, low salinity and low density. The ore-forming materials is muti-source and is mainly mantle-derived fluid, magmatic water and meteoric water. The ore-forming depth is large, and the denudation thickness is small, so the deep prospecting potential is great.The Shaling deposit has deep metallogenic depth and small denudation, therefor keeps great Prospecting Prospects in the deep.