In order to improve the driving safety and driving comfort of the expressway tunnel entrance, the driver may be temporarily blind due to the variation of the light intensity at the entrance of the expressway tunnel. Based on the theories of traffic engineering and cardiophysiology, the parameters of physiological reaction, driving speed, light intensity and GPS data of drivers entering the tunnel are detected by driving test. The relationship between the distance of highway tunnel entrance and light intensity, speed and driver's psychophysiological reaction is analyzed qualitatively and quantitatively, and the multiple regression safety evaluation model of tunnel entrance section is established. The results show that: drivers tend to slow down into the tunnel and accelerate to leave the tunnel, and the heart rate increases with the increase of the speed; the illumination changes most obviously from 3m to 20m outside the tunnel entrance, and the heart rate growth rate of drivers increases gradually with the decrease of illumination. The heart rate growth rate reaches the peak value at the position of 8m away from the tunnel entrance, with the maximum value of 31.95% The comfort threshold is [5%, 57%], which provides a theoretical reference for the design of lighting parameters and traffic safety management of highway tunnel entrance.