氮添加与凋落物管理对华北落叶松人工林土壤真菌群落的短期影响
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S714

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“十三五”国家重点研发计划项目子课题(2017YFD060040303);内蒙古自治区自然科学(2018MS03037) 第一作者:王志波(1984-),男,蒙古族,内蒙古赤峰人,副研究员。研究方向:森林培育。E-mail: wang_zb123@163.com。*通讯作者:季蒙(1962-)男,汉族,江苏南通人,研究方向:森林培育。E-mail: jimeng04@tom.com。 *,张海东1,李永乐2,李银祥1


Short-term Effects of Nitrogen Addition and Litter Management on Soil Fungal Community in Larix principis-rupprechtii Plantation
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    摘要:

    本文以阴山山地华北落叶松人工林为研究对象,开展氮添加和凋落物管理试验,利用高通量测序技术,研究不同措施对表层土壤(0~10cm)真菌群落组成、多样性和功能的短期影响,并对二者交互作用进行探讨。结果表明:氮添加和凋落物管理显著增加子囊菌门相对丰度,降低担子菌门相对丰度。氮添加显著增加糙缘腺革菌属相对丰度,高氮最大,为10.15%;凋落物管理显著增加猪块菌属、马鞍菌属和被孢霉菌属相对丰度,均堆积最大,分别为11.03%、13.57%和6.36%。两种处理方式均显著提高土壤真菌Shannon指数和Simpson指数,分别高氮、均搅拌最大,结果为6.41、5.77和0.96、0.94。氮添加和凋落物管理显著降低共生营养型真菌相对丰度。土壤pH和硝态氮含量是影响土壤真菌群落的主要因子。高氮搅拌土壤有效磷、速效钾、硝态氮含量以及真菌OTU数目、Alpha多样性指数、腐生营养型真菌相对丰度均最大,对促进凋落物分解,维持地力平衡具有重要作用。

    Abstract:

    In the study, the experiment with nitrogen addition treatment and litter management were carried out in Larix principis-rupprechtii plantation of Yin Mountains. The short-term effects of fungal community composition, diversity and function in surface soil (0~10cm) were analyzed by high-throughput sequencing technology. The interaction of nitrogen addition and litter management were studied. The results showed that nitrogen addition and litter management significantly increased the relative abundance of Ascomycota, but the relative abundance of Basidiomycota was significantly reduced. Nitrogen addition significantly increased the relative abundance of Amphinema, with high nitrogen being the largest was 10.15%. Litter management significantly increased the relative abundance of Choiromyces, Helvella and Mortierella, with the largest amount in gathering management were 11.03%、13.57% and 6.36%. The two treatments significantly increased the Shannon index and Simpson index of soil fungi, with the largest amount were 6.41, 5.77 and 0.96, 0.94 in high nitrogen and mixing. Nitrogen addition and litter management significantly reduced the relative abundance of Symbiotroph. Soil pH and nitrate nitrogen content are the main factors affecting soil fungal community. The contents of soil available phosphorus, available potassium, nitrate nitrogen, the number of OTU, alpha diversity index, and the relative abundance of Saprotroph in high nitrogen and mixing measure were all the highest. The research results played an important role in promoting the decomposition of litter and maintaining the balance of soil fertility.

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王志波,季蒙,张海东,等. 氮添加与凋落物管理对华北落叶松人工林土壤真菌群落的短期影响[J]. 科学技术与工程, 2021, 21(23): 9790-9798.
Wang Zhibo, Ji Meng, Zhang Haidong, et al. Short-term Effects of Nitrogen Addition and Litter Management on Soil Fungal Community in Larix principis-rupprechtii Plantation[J]. Science Technology and Engineering,2021,21(23):9790-9798.

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  • 收稿日期:2020-11-19
  • 最后修改日期:2021-06-04
  • 录用日期:2021-04-16
  • 在线发布日期: 2021-08-31
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