The 8m × 6m test section of large-scale low-speed wind tunnel can reduce the scale effect of the model and better simulate the details of the tested model, which has a wide range of application needs in the fields of aviation and aerospace. The larger the size of wind tunnel, the higher the requirements on the safety, reliability and construction cost of the wind tunnel structure. Therefore, it is necessary to carry out structural strength and stiffness analysis on the wind tunnel test section in the design stage. In the article, the finite element simulation analysis of 8m×6m test section structure of large low-speed wind tunnel based on TSV software were carried out. According to the results, the test section structure was optimized and improved, and the optimized results were analysed and optimized. The strength and stiffness of the overall structure of the 8m×6m test section structure of large low-speed wind tunnel could meet the engineering requirements under various loading conditions. The calculated modal results were consistent with the original design scheme, which verified the rationality and reliability of the analysis model.