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马强,夏冬双,武志华,等. 内蒙古西部地区土壤真菌的多样性分析[J]. 科学技术与工程, 2020, 20(35): 14447-14454.
Ma Qiang,et al.Analysis on the Diversity of Soil Fungi in the Western Region of Inner Mongolia[J].Science Technology and Engineering,2020,20(35):14447-14454.
内蒙古西部地区土壤真菌的多样性分析
Analysis on the Diversity of Soil Fungi in the Western Region of Inner Mongolia
投稿时间:2019-11-20  修订日期:2020-08-28
DOI:
中文关键词:  PCR-DGGE  土壤  真菌  多样性  内蒙古西部地区
英文关键词:PCR-DGGE  soil  fungi  diversity  the western region of Inner Mongolia
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
                    
作者单位
马强 内蒙古农业大学
夏冬双 内蒙古农业大学
武志华 内蒙古农业大学
王雪寒 内蒙古农业大学
任兴波 内蒙古农业大学
丁一秀 内蒙古农业大学
刘惠荣 内蒙古农业大学
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中文摘要:
      摘 要:为明确内蒙古西部地区土壤真菌的多样性,从内蒙古西部地区采集土壤样品170份,提取土壤样品的总DNA,通过聚合酶链反应-变性梯度凝胶电泳PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis),对真菌的丰富度和香浓指数进行分析,并研究了土壤真菌多样性与土壤类型、土壤利用方式及土壤环境参数的相关性。结果显示,内蒙古西部地区土壤真菌的多样性较为丰富,丰富度指数处于11到25.4之间,香浓指数处于2.33到3.17之间。在内蒙古西部同一地区的不同类型土壤中的真菌多样性差异显著。其中,棕钙土、栗钙土、石质土的丰富度最高,草甸土的最低;同一地区相同类型土壤中草地的真菌数量比耕地丰富,林地土壤真菌数量相对较少,未利用土壤真菌多样性指数最低。真菌多样性与土壤环境参数的相关性分析结果显示二者之间并无显著的相关性。经过18S rRNA 基因序列比对共得到了9个属的真菌,Penicillium(青霉属)、Aureobasidium(短柄霉属)、Pyrenochata(棘壳菌属)、Rhodocybella(红盖菇属)、Vanderwaltozyma、Kazachstania、Cystofilobasidium、Saccharomyces(酵母菌属),还有一株未被培养的优势菌。内蒙古西部地区的真菌资源较为丰富,其多样性受土壤类型及利用方式影响较大,作为与人类活动息息相关的一种重要的微生物资源,本研究为今后该地区真菌资源的开发利用奠定了基础。
英文摘要:
      Abstract: The purpose of this study was to determine the diversity of soil fungi in the western region of Inner Mongolia. 170 soil samples were collected from the western region of Inner Mongolia. The total DNA was extracted from the soil samples and the richness and aroma index of fungi were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Then, the relationship between the diversity of soil fungi and soil types, soil utilization patterns, and soil environmental parameters were studied. The results showed that the diversity of soil fungi in the western region of Inner Mongolia was relatively rich. The richness index of the soil samples was between 11 and 25.4 and the Shannon index was between 2.33 and 3.17. The diversity of fungi in different types of soil in the same area was quite different. Among them, the richness of fungi of brownpedocals, Castanozems, and Litho Soils was the highest, while that of Meadow soils was the lowest. The amount of fungi in grassland was more abundant than that in cultivated land in the same type of soil in the same area, while the number of fungi in forest soil was relatively low and the diversity index of fungi of unused soil was the lowest. The correlation analysis between the forest soil was relatively low and the diversity index of fungi of unused soil was the lowest. The correlation analysis between the diversity of fungi and soil environmental parameters showed that there was no significant correlation between them. A total of 9 genera of fungi were obtained by 18S rRNA gene sequence analysis, including Penicillium, Aureobasidium, Pyrenochata, Rhodocybella, Vanderwaltozyma, Kazachstania, Cystofilobasidium, Saccharomyces and an uncultured fungus. In conclusion, the fungus resources in the western region of Inner Mongolia are abundant and the diversity of fungi in this areais greatly affected by soil types and soil utilization patterns. This study laid a foundation for further research and development and utilization of fungi resources in the western region of Inner Mongolia.
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