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张涛涛,张 虹,赵娟,等. 黄赭色链霉菌11F发酵工艺及其对黄瓜幼苗生长的影响[J]. 科学技术与工程, 2020, 20(16): 6391-6396.
zhangtaotao,et al.Effect of Streptomyces silaceus 11F on growth of cucumber seedlings and control effect against Fusarium Wilt[J].Science Technology and Engineering,2020,20(16):6391-6396.
黄赭色链霉菌11F发酵工艺及其对黄瓜幼苗生长的影响
Effect of Streptomyces silaceus 11F on growth of cucumber seedlings and control effect against Fusarium Wilt
投稿时间:2019-11-06  修订日期:2020-06-16
DOI:
中文关键词:  链霉菌  黄瓜枯萎病  促生  防效
英文关键词:Streptomyces, biocontrol, growth promoting, cucumber wilt  
基金项目:北京市自然科学基金面上项目(6192008);国家重点研发计划项目(2018YFD0201300);北京市农林科学院创新专项(KJCX20200429)
              
作者单位
张涛涛 北京市农林科学院植物保护环境保护研究所
张 虹 北京市农林科学院植物保护环境保护研究所
赵娟 北京市农林科学院植物保护环境保护研究所
田兆丰 北京市农林科学院植物保护环境保护研究所
吴慧玲 北京市农林科学院植物保护环境保护研究所
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中文摘要:
      为了深入研究黄赭色链霉菌对黄瓜生长及黄瓜枯萎病的防治效果,本文采用固体发酵单因素方差分析对菌株11F的固体发酵条件(种龄、配水比、接种量、发酵基质)进行了优化,通过检测存活率测定在不同条件处理下菌剂链霉菌孢子对菌剂耐热力与抗紫外作用进行了分析,通过温室盆栽实验研究菌株11F菌剂对黄瓜生长的影响及对黄瓜枯萎病的防治效果。结果表明:最佳固体发酵基质配比为80%大米+10%麦麸,最佳料比水为1:0.3,最佳种龄为60 h,最佳接种量为10%。通过筛选得到菌剂最适载体是壳聚糖,温室盆栽实验显示菌株11F的发酵液与固体菌剂对黄瓜的生长都有促生作用。菌剂11F与蛭石的比例为1:100时黄瓜幼苗的株高、根长、鲜重、干重都显著高于其它处理,株高增加39%,根长增加了33.9%,鲜重增加了35.3%,干重增加了40.9%,其长势茂盛,叶色浓绿,促生效果明显。菌剂11F对黄瓜枯萎有明显的防治效果,防效为73.45%,由此说明菌株11F菌剂可用于黄瓜枯萎的防治。
英文摘要:
      In order to study the effect of Streptomyces silaceus on cucumber growth and Fusarium wilt control, the single factor variance analysis of solid fermentation was used to optimize the solid fermentation conditions of strain 11F (seed age, water ratio, inoculum amount, fermentation substrate). The effect of Streptomyces silaceus spores on the heat resistance and UV resistance was tested under different conditions. The effects of strain 11F on the growth of cucumber and the control of cucumber fusarium wilt were studied by pot experiment in greenhouse. The results showed that the best solid fermentation medium was 80% rice + 10% wheat bran, the best ratio of material to water was 1:0.3, the best seed age was 60 h, and the best inoculation amount was 10%. Chitosan was the most suitable carrier of the fungicide. The greenhouse pot experiment showed that the solid fungicide of strain 11F could promote the growth of cucumber. When the ratio of 11F and vermiculite was 1:100, the plant height, root length, fresh weight and dry weight of cucumber seedlings were significantly higher than those of other treatments. The plant height, root length, fresh weight and dry weight increased by 39%, 33.9%, 35.3% and 40.9%, respectively. The growth of cucumber seedlings was vigorous, the leaves were green, and the growth promoting effect was obvious. The control effect of 11F on fusarium wilt of cucumber was 73.45%, which indicated that 11F could be used in the control of cucumber wilt.
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