Walking, as an important means of daily transportation, and also the most important way for teachers and students to travel on campus, has obvious ecological, social, economic and health benefits. The research on walkability evaluation of university campus in cold region is helpful to optimize the walkability environment, enhance campus vitality, and improve the health level of teachers and students. Based on the influence factors of walking demand and walkability of university campus in cold region, and used AHP- fuzzy comprehensive evaluation method, fuzzy Delphi method, the fuzzy comprehensive evaluation model for walkability of university campus in cold region was built from the facility level, environment level and run level. MATLAB, EXCEL and other software were used to calculate the fuzzy comprehensive evaluation model, and three universities in Harbin were evaluated. The result of the walkability evaluation of the three university campuses shows that Harbin Institute of Technology > Heilongjiang University > Northeast Agricultural University. Finally, the corresponding optimization strategies are proposed for the existing problems in the construction of the pedestrian environment, so as to provide reference for the research on the walkability of university campuses in cold regions.