In order to study the risk level of vehicle operation, the fuzzy set theory and DS evidence fusion algorithm were used to study the probability membership values of different factors at different risk levels. The analytic hierarchy process was used to calculate the weight value of different factors to the vehicle operation risk system. Then the probability of different levels of risk and comprehensive risk value arising from the comprehensive factors of vehicle operation were calculated. In the case study, one typical freeway section in Hunan province was selected. The calculation results showed the vehicle on the study section is in medium-level risk. Therefore, the fuzzy set-DS evidence theory-AHP method is effective for vehicle operation risk assessment.